spodoptera frugiperda 9 sf 9 insect cells Search Results


insect cells sf9 (spodoptera frugiperda 9)  (BioResource International Inc)
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BioResource International Inc insect cells sf9 (spodoptera frugiperda 9)
Lack of HBc secretion of an HBc double mutant L65A/V89A in human hepatocytes and insect cells. A A cartoon illustration of secreted HBV particles, including virions, naked capsids, and HBsAg particles. Left panel: genome-containing and genome-free empty virions contain both surface antigen and core proteins; middle panel: genome-containing and genome-free naked capsids contain no surface antigens; right panel: HBV surface antigen particles contain no core protein. B Upper panel: Two CRM1-dependent nuclear export signals (HBc NES CRM1 -I and NES CRM1 -II) were mapped to the tip of the spike of HBc particles . The double mutations L65A/V89A at NES I + II are shown in red color. Lower panel: No secreted naked capsids were detected by ELISA in the media of <t>Sf9</t> insect cells transfected with an HBc mutant L65A/V89A expression vector (left panel). Nor can any naked capsids be detected in the media of HuH-7 human hepatocytes transfected with an HBV replicon (right panel). The ELISA values were calculated from three independent repeat experiments. WT: wild type HBV. Mock: un-transfected cells
Insect Cells Sf9 (Spodoptera Frugiperda 9), supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
insect cells sf9 (spodoptera frugiperda 9) - by Bioz Stars, 2026-10
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Lack of HBc secretion of an HBc double mutant L65A/V89A in human hepatocytes and insect cells. A A cartoon illustration of secreted HBV particles, including virions, naked capsids, and HBsAg particles. Left panel: genome-containing and genome-free empty virions contain both surface antigen and core proteins; middle panel: genome-containing and genome-free naked capsids contain no surface antigens; right panel: HBV surface antigen particles contain no core protein. B Upper panel: Two CRM1-dependent nuclear export signals (HBc NES CRM1 -I and NES CRM1 -II) were mapped to the tip of the spike of HBc particles . The double mutations L65A/V89A at NES I + II are shown in red color. Lower panel: No secreted naked capsids were detected by ELISA in the media of Sf9 insect cells transfected with an HBc mutant L65A/V89A expression vector (left panel). Nor can any naked capsids be detected in the media of HuH-7 human hepatocytes transfected with an HBV replicon (right panel). The ELISA values were calculated from three independent repeat experiments. WT: wild type HBV. Mock: un-transfected cells

Journal: Journal of Biomedical Science

Article Title: Hepatitis B virus virion secretion is a CRM1-spike-mediated late event

doi: 10.1186/s12929-022-00827-w

Figure Lengend Snippet: Lack of HBc secretion of an HBc double mutant L65A/V89A in human hepatocytes and insect cells. A A cartoon illustration of secreted HBV particles, including virions, naked capsids, and HBsAg particles. Left panel: genome-containing and genome-free empty virions contain both surface antigen and core proteins; middle panel: genome-containing and genome-free naked capsids contain no surface antigens; right panel: HBV surface antigen particles contain no core protein. B Upper panel: Two CRM1-dependent nuclear export signals (HBc NES CRM1 -I and NES CRM1 -II) were mapped to the tip of the spike of HBc particles . The double mutations L65A/V89A at NES I + II are shown in red color. Lower panel: No secreted naked capsids were detected by ELISA in the media of Sf9 insect cells transfected with an HBc mutant L65A/V89A expression vector (left panel). Nor can any naked capsids be detected in the media of HuH-7 human hepatocytes transfected with an HBV replicon (right panel). The ELISA values were calculated from three independent repeat experiments. WT: wild type HBV. Mock: un-transfected cells

Article Snippet: Insect cells Sf9 ( Spodoptera frugiperda 9 ) were purchased from BCRC (Bioresource Collection and Research Center, Taiwan; ATCC number: CRL-1711).

Techniques: Mutagenesis, Enzyme-linked Immunosorbent Assay, Transfection, Expressing, Plasmid Preparation